Preparation of Partially Hepatectomized Rats
نویسندگان
چکیده
invasive measurement of cellular proliferation, namely, liver regeneration; although they did not show the site of the regeneration. Although PET is noninvasive andcan be performedon a living animal in real time, identifying the precise sites of synthesis is difficult. Thus, we determined site-specific pro tein and DNA syntheses precisely with a bioimaging ana lyzer system (BAS), which, although an invasive method, enables identificationof precise sites.
منابع مشابه
Comparative studies on bile flow and biliary lipid excretion after bile-acid loading in normal and partially hepatectomized rats.
This study was performed to investigate sequential changes in bile secretion and biliary lipids after taurocholic acid (TCA) loading of regenerating rat liver. TCA was administered intravenously at stepwise-increasing doses to groups of non-operated control and partially hepatectomized rats, 24, 72 and 168 h after surgery. Bile flow, bile-acid output (BAO) and phospholipid output (PLO) (express...
متن کاملEffect of exchange transfusion on labeling of nuclei with thymidine-3-H and on mitosis in hepatocytes of normal and regenerating rat liver.
Exchange transfusion of blood (75-80% replacement of blood of recipient animals) was used to study the effect of blood-borne influences on nuclear labeling with thymidine-3H and on occur rence of mitosis in hepatocytes of rat liver. Intact or shamhepatectomized rats and partially hepatectomized rats were: (a) not transfused, (6) transfused with blood from rats with intact livers, or (c) transfu...
متن کاملSerum from partially hepatectomized rats induces primary hepatocytes to enter S phase: a role for prostaglandins?
Serum obtained from partially hepatectomized rats 1, 3 or 24 h after operation was more effective in stimulating DNA synthesis in primary adult rat hepatocytes than serum from sham-operated rats; exposure to the serum for 2 h was sufficient to promote growth. Serum from the partially hepatectomized rats contained elevated levels of PGE2 and PGF2 alpha; it promoted hepatocytes to release prostag...
متن کاملDistribution of biotrace elements in partially hepatectomized rats
The effect of liver regeneration on intestinal absorption and tissue distribution of biotrace elements was investigated by the multitracer technique. In the liver of partially hepatectomized rats, it was observed that the amount of Na significantly increased after hepatectomization, while the amounts of V, Cr, Sr, Zn, and Mn were transiently high in the liver 1 hour after hepatectomization, and...
متن کاملOn the Regenerative Process of the Hepatic Parenchymal Cells in Parabiotic Rats.
In order to investigate on humoral factors concerning regeneration and proliferation of cells, histological, histochemical and micrometrical observations were made on livers of successful parabiotic rats, at intervals during regenerative course of one of the partners which had been partially hepatectomized. Changing pattern in size of hepatic cells was very similar between hepatectomized and no...
متن کاملDifferential mechanisms of increased alpha 1-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy.
Possible differences in the mechanisms of increased alpha1-fetoprotein (AFP) production following carbon tetrachloride (CCl4) intoxication and partial hepatectomy were studied with 5-week-old rats at the time of sacrifice. The maximum level of serum AFP reached in 4 days after a single dose of CCl4 was much higher than that after partial hepatectomy, although the incorporation of [3H]thymidine ...
متن کامل